Cellulase from Ruminococcus albus and Mixed Rumen Microorganisms

Preparation of the cellulase from the cellulolytic anaerobic rumen bacterium Ruminococcus albus and its release from the bacterial cell wall.

1. Most of the cellulase (CM-cellulase) elaborated by the rumen bacterium Ruminococcus albus strain SY3, which was isolated from a sheep, was cell-wall-bound. 2. The enzyme could be released readily by washing either with phosphate buffer or with water. 3. The amount of enzyme released was affected by the pH and ionic strength of the phosphate buffer. 4. The cell-wall-bound enzyme was of very h...

Functional analyses of multiple lichenin-degrading enzymes from the rumen bacterium Ruminococcus albus 8.

Ruminococcus albus 8 is a fibrolytic ruminal bacterium capable of utilization of various plant cell wall polysaccharides. A bioinformatic analysis of a partial genome sequence of R. albus revealed several putative enzymes likely to hydrolyze glucans, including lichenin, a mixed-linkage polysaccharide of glucose linked together in β-1,3 and β-1,4 glycosidic bonds. In the present study, we demons...

Nutritional Interdependence Among Rumen Bacteria, Bacteroides amylophilus, Megasphaera elsdenii, and Ruminococcus albus.

Nutritional interdependence among three representatives of rumen bacteria, Bacteroides amylophilus, Megasphaera elsdenii, and Ruminococcus albus, was studied with a basal medium consisting of minerals, vitamins, cysteine hydrochloride, and NH(4). B. amylophilus grew well in the basal medium supplemented with starch and produced branched-chain amino acids after growth ceased. When cocultured wit...

Utilization of Ruminal Epithelial Cells by Ruminococcus albus, with or without Rumen Protozoa, and Its Effect

Effects of supplementation with ruminal epithelial cells on fiber-degrading activity and cell growth of Ruminococcus albus (R. albus, strain 7) was tested using a basal substrate of rice straw and formulated concentrate. Cultures of R. albus alone and R. albus with rumen protozoa were grown at 39C for 48 h with an 8.4% crude protein (CP) substrate, 33% of the CP supplemented with either rumina...

Cellulose Adherence Factors in Ruminococcus albus